Figure 2.

DCs cross-present antigen in an immunogenic fashion upon stimulation with Ad encoding TAs or PeptiCRAd. Mouse dendritic cell line JAWS II was pulsed with Ad5/3Δ24, peptide alone (polyKSIINFEKL), AdOVA, PeptiCRAd-SIINFEKL or left unpulsed (cells only). The viruses were used at 250 MOI. Flow cytometry analysis was used to determine the cross-presentation at 24h (A) and 48h (B) post incubation. CD86 was used to measure DCs activation and an antibody specific for OVA peptide SIINFEKL complexed with H2Kb to detect the antigen presentation. The data are depicted as bar plot mean ± SEM. Statistical analysis was performed with ordinary One-way ANOVA (ns P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, P ≤ 0.0001).