Fig. 2.

PTN is a direct target of miR-384 in osteosarcoma. (A) The endogenous expression levels of miR-384 in OS cell lines were analyzed by quantitative RT-PCR (U6 primer was used as the reference, and miR-384 expression in MG63 cells was used as the baseline). (B) miR-384 was predicted to bind to the 3′-UTR of PTN mRNA according to TargetScan analysis. (C) Effect of luteolin on the expression of miR-384 in MG63,MG63/DOX and U2OS cells. (D) Effect of luteolin on PTN expression in OS cells. (E) Expression of miR-384 was examined by qRT-PCR after transfected with miR-384 mimic, miR-384 inhibitor or negative control. (F) A luciferase assay was used to determine whether miR-384 directly targeted PTN. NC, negative control; WT, wide type; MUT, mutant.