Table 1.
qPCR primer name | Sequence (5′-3′) |
miR-384 (Forward) | TGTTAAATCAGGAATTTTAA |
miR-384 (Reverse) | TGTTACAGGCATTATGAA |
PTN (Forward) | GGGGAGAATGTGACCTGAAC |
PTN (Reverse) | AGGGCTTGGA GATGGTGA |
β-actin (Forward) | GTGGACATCCGCAAAGAC |
β-actin (Reverse) | AAAGGGTGTAACGCAACTA |
U6 (Forward) | CTCGCTTCGGCAGCACA |
U6 (Reverse) | AACGCTTCACGAATTTGCGT |
mimic/inhibitor name | Sequence (5′-3′) |
PTN shRNA | TCAAGCAGAATCTAAGAAG |
miR-384 mimic | AUUCCUAGAAAUUGUUCAUA |
miR-384 inhibitor | UAUGAACAAUUUCUAGGAAU |
miR-384 mimic NC | UUCUCCGAACGUGUCACGU |
miR-384 inhibitor NC | CAGUACUUUUGUGUAGUACAA |