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. 2022 Apr 20;19:237–250. doi: 10.1016/j.bioactmat.2022.04.011

Fig. 2.

Fig. 2

The evaluation of CDT and PTT in vitro. (a) The images of different reaction systems. (b) The absorbance of different groups at 663 nm (G1: MB, G2: MB + H2O2, G3: MB + GSMM, G4: MB + GSMM + H2O2, G5: MB + GSMM + H2O2 + NaHCO3). (c) The cell viability with different concentration GSMM co-incubation (0, 5, 10, 20, 40, 80 μg/mL) after laser irradiation (1064 nm, 0.6 W/cm2) in different treatment time (Two-way ANOVA with Tukey's multiple comparisons test; ns: no significance, *: p < 0.05, **: p < 0.01, ***: p < 0.001). (d) Immunofluorescence images of ROS staining (DCFH-DA probe) of B16/F10 cells after different concentration GSMM co-incubation (0, 5, 10, 20, 40 μg/ml) treatments with or without laser irradiation (Scale bar: 50 μm). (e) Immunofluorescence images of live/dead staining (Calcein acetoxymethyl ester and propidium iodide probes) of B16/F10 cells with or without nanoparticles (GSM or GSMM, 80 μg/mL) co-incubation under 1064 nm laser irradiation treatment (0.6 W/cm2, 5 min). Scale bar: 200 μm. (f) Immunofluorescence images of CRT expression on B16/F10 with or without nanoparticles (GSM or GSMM, 80 μg/ml) co-incubation under a 1064 nm laser irradiation treatment (0.6 W/cm2, 5 min). Scale bar: 50 μm.