Proparacaine on corneal stromal fibroblast migration. Phase-contrast microscopy images show the effect of regular (0.5%) and diluted (0.05%) proparacaine on the hCSF migration rate with or without an antibiotic environment conducted through scratch assay. The experiments were performed in triplicate. Cultures showing the scratch wound at 0 h in −TGFβ1 (A), scratch wound at 0 h in +TGFβ1 (B), hCSF migration in diluted proparacaine and −TGFβ1 at 24 h (C), hCSF migration in diluted proparacaine and +TGFβ1 at 24 h (D), hCSF migration in regular proparacaine and −TGFβ1 at 24 h (E), and hCSF migration in regular proparacaine and +TGFβ1 at 24 h (F). Scale bar = 100 μm. Color images are available online.