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. 2022 Apr 14;13:871624. doi: 10.3389/fmicb.2022.871624

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Copyright © 2022 Ohtake, Kawase, Pontrelli, Nitta, Laviña, Shen, Putri, Liao and Fukusaki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PCA analysis of SYN3 and SYN12. The score plot derived from autoscaled (A) GC/MS data. (B) Ion-pair LC/MS/MS data. The corresponding loading plot illustrating metabolites that contributed to the separation on PC1 and PC2 derived from autoscaled. (C) GC/MS data. (D) Ion-pair LC/MS/MS data. All metabolites contribution is shown in Supplementary Figure 1. (E) The result of intracellular key intermediate, by-products and 1-propanol production. Metabolite intensities shown in the y-axis were normalized to an internal standard. Asterisks indicate significant difference from the strain (*p < 0.05, **p < 0.01). The error bars indicate standard deviations obtained from three replicate fermentations.