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. Author manuscript; available in PMC: 2022 Apr 28.
Published in final edited form as: Biochem Pharmacol. 2020 Jan 3;174:113789. doi: 10.1016/j.bcp.2019.113789

Table 1.

SOX9 Targeting miRNAs in Human Breast Cancer

miRNA Patient samples In-vitro model In-vivo model Functions Refs
miR-140 43 tumor samples and 5 normal samples MCF10A, MCF10DCIS, SUM225CWN and SUM102PT cells Immuno-deficient nude female mice miR-140 is significantly downregulated in cancer stem-like cells and enforced expression reduced CSC self-renewal and tumor formation by directly targeting ALDH1 and SOX9 [47]
miR-3134 MCF7 cells miR-3134 (ARE binding miRNA) induced the expression of SOX9 in collaboration with ARE binding protein HuR [91]
miR-140 MCF10DCIS and mouse preadipocytes (3T3L1, MBA1) Immuno-deficient Nu/Nu female mice miRNA-140 was upregulated after shikonin treatment and reduced the expression of SOX9 [92]
miR-206 40 tumor samples MCF7, MDA-MB361, MDA-MB231, MDA-MB435, and HCC1395 miR-206 expression was downregulated in TNBC. Ectopic expression of miR-206 inhibited cell invasion and angiogenesis of TNBC by downregulating the expression of VEGF, MAPK3, and SOX9 [14]
miR-155 MCF7 and MDA-MB231 miR-155 was induced in exosomes isolated from CSCs and resistant cells and that exosomes induced the expression of SOX9 [93]
miR-133b 38 paired tissues BT549, MDA-MB231, BT474, SKBR3, HCC1937 BALB/c female nude mice, SCID/beige mice miR-133b was downregulated in tumor and associated with advanced grade. Forced expression of miR-133b inhibited cell growth, invasion both in-vitro and in-vivo by directly modulating SOX9 [94]
miR-511 51 pair MDA-MB231, MCF7 BALB/C nude mice miR-511 was downregulated in BC tissues and cell lines and associated with lymph node metastasis and tumor stage. Ectopic expression inhibited cell growth and metastasis invite and also attenuated tumor growth in-vivo by directly targeting SOX9 mediated PI3K/Akt pathway [95]
miR-190 30 paired tissues T47D, MCF7, MDA-MB231, MDA-MB435, MDA-MB468 Female BALB/c nude mice miR-190 induced TAM sensitivity of BC cells both in-vitro and in-vivo by targeting SOX9 resulted introverted Wnt/β-catenin signaling. The expression of miR-190 inversely correlated with SOX9 in BC samples [96]
miR-9-5p, miR-195-5p, and miR-203a-3p 12 pre and post NT MDA-MB231, MCF7, BT474 NOD/SCID/IL2R γ-null (NSG) mice Chemotherapy induced EV miRNA which targeted ONECUT2 resulting induction of CSC phenotype through induction of NOTCH1, SOX9, NANOG, OCT4, and SOX2 [97]
miRNA-215-5p 39 pair of breast carcinoma tissues MCF10A, MDA-MB468, MDA-MB231 and MCF7 BALB/c nude mice miR-215-5p was downregulated in BC tissue samples. Upregulation of miR-215-5p inhibited BC cells growth and metastasis both in-vitro and in-vivo by directly targeting SOX9 [98]

BC, breast cancer; TNBC, triple negative BC; NT, neoadjuvant therapy