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. 2021 Oct 20;32(3):e13028. doi: 10.1111/bpa.13028

FIGURE 7.

FIGURE 7

Double immunostaining of TH and p‐Tau Thr231 in LC of 6–6.5 months old mice after CSD. The double immunostaining of TH and p‐Tau Thr231 in mouse LC (A). Integrated density of TH staining normalized to Hoechst staining was analyzed, n = 4 mice in each group (a‐1). Integrated density of p‐Tau Thr231 staining normalized to Hoechst staining was analyzed, n = 4 mice in each group (a‐2). The percentage of TH and p‐Tau Thr231 co‐stained cells in mouse LC after CSD, n = 4 mice in each group (a‐3). Scale bar: 200μm. Data were the mean ± SEM values. *p < 0.05, **p < 0.01, ***p < 0.001, by two‐way ANOVA with Tukey's multiple comparisons test.