Table 1. Number of specimens belonging to 19 insect orders from Pakistan with DNA barcode records. The number of families, genera, species, and BINs is reported for each order.

Order	Specimens with barcodes	Specimens assigned to BINs (%)	BINs recovered	OTUs without BIN*	Singleton BINs (%)	BINs assigned to family (%)	Families recovered	BINs assigned to genus (%)	Genera recovered	BINs assigned to species (%)	Species recovered
Blattodea	64	84	19	5	36.8	100	5	78.9	9	52.6	10
Coleoptera	3,889	93	819	123	45.2	100	56	21.9	118	13.3	119
Dermaptera	24	83.3	3	2	33.3	100	2	33.3	1	0.0	0
Diptera	20,095	99	1,684	94	40.1	99.0	68	29.8	212	13.8	222
Embioptera	28	96.4	7	1	14.3	100	2	14.3	1	14.3	1
Hemiptera	5,859	96.5	642	73	41.9	98.3	59	31.6	132	22.6	135
Hymenoptera	10,542	96	1,711	177	47.7	99.4	50	34.7	226	10.2	170
Lepidoptera	6,064	99.4	1,233	24	42.5	99.6	62	71.9	516	41.5	514
Mantodea	113	97.3	36	2	50.0	100	2	13.9	4	5.6	2
Megaloptera	6	100	1	0	0.0	100	1	100	1	100	1
Neuroptera	559	92.3	99	6	39.4	99.0	7	54.5	30	36.4	32
Odonata	353	92.6	51	11	21.6	100	12	92.2	30	88.2	47
Orthoptera	1,409	97.59	163	21	30.1	100	12	44.2	53	37.4	54
Phasmatodea	4	75	3	1	100.0	100	1	0.0	0	0.0	0
Psocodea	950	97.5	31	5	22.6	93.5	13	38.7	10	19.4	6
Strepsiptera	2	100	1	0	0.0	100	1	100	1	0.0	0
Thysanoptera	618	99.3	76	2	34.2	100	3	80.3	27	69.7	48
Trichoptera	11	100	10	0	90.0	100	6	60.0	4	40.0	4
Zygentoma	2	100	1	0	0.0	0.0	0	0.0	0	0.0	0
Total	50,592	97.6%	6,590	547	42.9%	99%	362	40%	1,375	21%	1,364

Note:

^*For recognition as a new BIN, a sequence must include >500 bp of the barcode region (positions 70 bp to 700 bp in the BOLD alignment) and possess <1% ambiguous bases.