Figure 2.

(A) Plot of ΔG_hingevs number of complementary bases for nDFS.B S1, computed using the Boltzmann weight of the probability of the closed state as measured by the FRET ensemble data. (B) Plot of ΔG_DNA, the difference in free energy between the S1 DNA melted and annealed states vs length of complementary sequence computed using salt-adjusted free energies^33,34 with a reference concentration of 1 μM. (C) ΔΔG_N,N–1 computed separately from (A) ΔG_hinge or (B) ΔG_DNA, where ΔΔG_N,N–1 is the difference between ΔG_N and ΔG_N–1, which is the additional stability imparted by the addition of one base pair. The lower ΔΔG_N,N–1 for the DNA alone relative to the nDFS.B implies that the device acts on the oligonucleotides to alter the free energy of binding.