FIG. 3.
Effect of exogenous iron supplementation on the activity of CQ against intracellular growth of P. marneffei. P. marneffei conidia were incubated with J774 cells for 2 h at a 2:1 ratio in triplicate. After removal of nonphagocytized conidia, cultures were treated with CQ in the presence of FeNTA (50 μg/ml), FeCl3 (250 μM), or ferric NH4 citrate (100 μg/ml) and incubated for a further 48 h. The data are expressed as mean CFU ± SEM recovered from triplicate cultures of a representative of three experiments.