FIGURE 6.

RT-qPCR validation on selected genes on BCG Denmark WT and ΔTbD1 under rolling and standing culture conditions. Log2 fold-change (log2FC) values were generated by comparing the expression of genes of BCG Denmark WT and ΔTbD1 strains under different culture conditions vs. WT under rolling condition control using 2 –ΔΔCt method, and expression levels of (A) mmpS6, (B) mmpL6, (C) cysK2, and (D) ctpV genes have shown. The data shown are means ± SD of gene expression from three independent biological replicates with duplicates, normalized with respect to 16S rRNA. Statistical significances of differences were calculated by Student’s t-test (*p < 0.05; ^**p < 0.01; ^***p < 0.001; ns, not significant) are shown.