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. 2022 Apr 14;13:817952. doi: 10.3389/fmicb.2022.817952

FIGURE 7.

FIGURE 7

Contribution of TbD1 on BCG Denmark to Copper sensitivity. (A) BCG Denmark WT (circles), ΔTbD1 (squares), and ΔTbD1:TbD1 (triangles) strains were grown at 37°C in standing Sauton’s liquid media, and CuSO4 was added. Cultures were challenged with 0 μM CuSO4 (black symbols) or 150 μM CuSO4 (red symbols). Absorbance at 600 nm (OD600) was measured for 2 weeks. The data are representative of three independent experiments. (B) Drop assay determining the Copper resistance of TbD1 mutants. Serial dilutions (10-fold) of BCG Denmark WT, ΔTbD1, and ΔTbD1:TbD1 cultures were spotted onto 7H11 plates containing 25, 100, or 150 μM CuSO4. Data are representative of two independent experiments with replicates.