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. 2022 Apr 14;13:817952. doi: 10.3389/fmicb.2022.817952

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Copyright © 2022 Ma, Farrell, Gonzalez, Browne, Nakajima, Suzuki and Gordon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BCG Denmark ΔTbD1 copper response. BCG Denmark ΔTbD1 expressed copper-responsive genes at a significantly lower level under copper stress in standing culture conditions. Fold-change values were generated by comparing the expression of genes of WT, ΔTbD1, and ΔTbD1:TbD1 BCG Denmark strains treated with 100 μM CuSO4 for 3 h (marked as X) or untreated culture conditions vs. WT untreated control using 2 –ΔΔCt method, and expression levels of (A) cysK2 gene and (B) ctpV gene have shown. The data shown are means ± SD of gene expression from three independent biological replicates with duplicates, normalized with respect to two reference genes ftsZ and fbpB. Statistical significances of differences were calculated by one-way ANOVA followed by the Dunnett’s post hoc test for multiple-comparisons (^**p < 0.01; ^***p < 0.001; ^****p < 0.0001; ns, not significant) are shown.