FIGURE 1.

DEX/lipo is specifically delivered into the macrophages in lungs. (A) Schematic of DEX/lipo, left, and size determination of DEX/lipo by dynamic light scattering (DLS), right. The mean diameter is close to 1,000 nm. (B) Co-immunofluorescence (Co-IF) staining was performed to confirm the co-localization of the epithelial cell adhesion molecule (EpCAM) (green) or F4/80 (red) with DiI (yellow). Mice were injected intranally with 20 μl of DiI-labeled liposomes, and lung tissues were dissected at 24 h post-injection. Scale bar; 20 μm. (C) Population percentages in the lungs of mice are shown as total cells compared to DiI⁺ cells. Representatives of two independent experiments are shown. (D) Flow-cytometry analysis of DiI⁺ cells. CD45⁺Ly6C⁺CD64^low monocytes population, CD45⁺Ly6C^–CD64⁺CD11c⁺ alveolar macrophages population, and CD45⁺Ly6C^–CD64⁺CD11b⁺ interstitial macrophages population are shown in the DiI/forward scatter (FSC) dot plot with gating for the DiI⁺ population.