Fig. 3. Effects of BPN on restoration of PA-induced insulin signaling. BPN-treated HepG2 cells were incubated with PA for 16 h prior to stimulation with 100 nM insulin for 30 min. Immunoblotting assay was used to determine the phosphorylated and non-phosphorylated protein levels of IRβ, IRS1 and Akt. β-Actin was used as loading control. *P < 0.05, **P < 0.01, ***P < 0.001 versus PA-treated group.
