Fig. 5. Antioxidant activities of BPN. (A) DPPH-scavenging activity of BPN. Various concentrations of BPN were incubated with DPPH at 25 °C for 30 min, absorbance was read at 519 nm. (B) Flow cytometry assay was used to determine the percentage of PA-induced ROS generation in HepG2 cells. Cells were treated with PA for 16 h in absence or presence of BPN. After incubation, cells were washed with PBS, loaded with probe DCFH-DA and ROS generation was evaluated. **P < 0.01 relative to control.
