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. 2022 Feb 14;34(5):1933–1956. doi: 10.1093/plcell/koac044

Figure 5.

Figure 5

Phenotypes and biochemical analysis of the zmbzip29;zmabi19 double mutants. A, Ear phenotypes of the wild type, self-pollinated zmabi19/+, zmbzip29, and self-pollinated zmbzip29/+;zmabi19/+. The white and red arrowheads indicate the monogenic zmabi19 and digenic zmbzip29;zmabi19 mutant seeds, respectively. Bar = 1 cm. B, Kernel width of the wild-type, single and double mutant seeds. Bar = 1 cm. C, Kernel length of the wild-type, single and double mutant seeds. Bar = 1 cm. D, Measurement of 100-kernel weight. Error bars represent SD of three biological replicates. Statistical significance was determined with Student’s t test. **P < 0.01. E, Longitudinal sections of the wild-type, single and double mutant seeds. Bar = 1 mm. F, SDS-PAGE analysis of zein (top panel) and nonzein (bottom panel) proteins in the wild-type, single and double mutant seeds. The size of each zein protein band is indicated beside it. M, markers; γ27, 27-kD γ-zein; α22, 22-kD α-zein; α19, 19-kD α-zein; γ16, 16-kD γ-zein; β15, 15-kD β-zein; δ10, 10-kD δ-zein. G, Quantification of zein and nonzein proteins in the wild-type, single and double mutant seeds. In total, five kernels were ground into fine flour for each replicate. Error bars represent SD of three biological replicates. Statistical significance was determined with Student’s t test. **P < 0.01.