Figure 8.

Symbiotic gene expression in the Ljnrt2.1 mutants. A–E, RT-qPCR analysis of LjCLE-RS2 (A), LjNF-YA (B), LjNF-YB (C), LjEPR3 (D), and LjNIN (E) expression in the WT and Ljnrt2.1-1 mutants. Plants were pretreated with 0 (−) or 10 mM KNO₃ (+) for 24 h before rhizobial inoculation. Roots at 3 dai with continuous treatment of 0 or 10 mM KNO₃ were collected (n = 3 independent pools of roots derived from three plants), and total RNA from the 3 dai roots was isolated for cDNA synthesis. The expression of LjUBQ was used as the reference. Error bars indicate sem. *P < 0.05 by a two-sided Welch’s t test.