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Reciprocal complementation assays of LjNLP4 and LjNLP1. A and B, Nodule number and nodulation phenotypes of transgenic hairy roots of the Ljnlp4-1 (A) and Ljnlp1 (B) mutants transformed with either the LjUBQpro:GUS, LjUBQpro:LjNLP4, or LjUBQpro:LjNLP1 constructs in the presence of 0 (−) or 10 mM KNO3 (+) at 21 dai (n = 6–11 plants). Transgenic roots were identified by GFP fluorescence. Error bars indicate sem. *P < 0.05 by a two-sided Welch’s t test. Scale bars = 1 mm.
