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. 2022 Feb 16;34(5):2056–2079. doi: 10.1093/plcell/koac056

Figure 3.

Figure 3

Relative accumulation of cytosolic and chloroplast rRNAs as a measure for the accumulation of the corresponding ribosomal subunits during cold acclimation. Bar plots showing the relative accumulation of rRNAs (normalized to 25S rRNA) from acclimating plants (12°C, blue) and control plants (24°C, green) at the indicated time points after cold shift. The accumulation of nuclear 25S rRNA and 18S rRNA, chloroplast 23S rRNAs (23SM1:∼1 kb; 23SM2:∼1.2 kb, representing two of the three hidden break products (Kössel et al., 1985)), and 16S rRNA were quantified with 2100 Agilent Bioanalyzer and displayed as rRNA ratios by normalizing to 25S rRNA. Error bars denote the standard deviation of results obtained from three individual plants used as independent biological replicates. Note that no statistically significant differences were observed between acclimating and control plants (Supplemental Data Set 1).