Figure 4.

Three homeoalleles of TaWRKY19 in wheat play redundant roles in regulation of ROS production and Pst resistance. A, Transgenic complementation assay using the three wheat homeoalleles of TaWRKY19 (TaWRKY19-2A, TaWRKY19-2B, and TaWRKY19-2D). tawrky19-A transgenic plants were transformed with each homeoallele individually (tawrky19-A°TaWRKY19-A, tawrky19-A°TaWRKY19-B, and tawrky19-A°TaWRKY19-D). The leaves were inoculated with Pst CYR33, which is virulent on the WT Fielder plants and examined at 14 dpi. B, Extracellular H₂O₂ contents in the plants from (A) at 24 hpi with Pst CYR33. Data are shown as means ± SD from three biological replicates. Statistical significance was determined by Student’s t test. *P < 0.05. **P < 0.01. ns, not significant. C, Fungal biomass at 14 dpi with Pst CYR33, as determined by qPCR. PsEF1 and wheat TaEF1α were used as the internal control gene to evaluate Pst and wheat DNA with reference to gene-specific standard curves. Data are shown as means ± SD from three biological replicates. Statistical significance was determined by Student’s t test. **P < 0.01.