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. 2022 Jan 31;34(5):2001–2018. doi: 10.1093/plcell/koac026

Figure 8.

Figure 8

SlVOZ1 directly binds to the SFT promoter to activate transcription. A, Illustration of the binding motifs of SlVOZ1 in the SFT promoter. B, EMSA showing the direct binding of SlVOZ1 to the SFT promoter probes. Recombinant GST-SlVOZ1, GST-SlOST1, or both proteins were incubated with FAM-labeled DNA fragments. C, Schematic diagrams of the effector and reporter constructs used for the transactivation assay. D, Transactivation assay showing that SlVOZ1 activates the transcription of SFT. **P < 0.01, Student’s t test, relative to WT. The protein abundance of effectors was detected by immunoblot using anti-GFP antibody. E, ChIP-qPCR assay showing the binding of SlVOZ1 to the SFT promoter in vivo. Leaves of 6-week-old tomato plants grown under LD conditions were harvested in the morning for ChIP. Data represent means ± sd of three technical repeats. *P < 0.05, **P < 0.01, Student’s t test, relative to WT. Anti-SlVOZ1 antibody was used to precipitate the SlVOZ1–DNA complex in WT and slvoz1 mutants. ChIP experiments were performed two independent times with similar results.