| GC-MS |
• Mature technology |
• Requires sample derivatization |
| • Comparatively cheap |
• Relatively long time for sample analysis |
| • Excellent separation reproducibility |
• Unable to produce parent ions |
| • Suitable for the detection of volatile metabolites |
• Novel compound identification is difficult |
| • Universal databases facilitate metabolite identification |
| LC-MS |
• Excellent sensitivity |
• Comparatively expensive |
| • Simple sample pretreatment process |
• Lower reproducibility than GC-MS |
| • Wide coverage of metabolite detection |
• Not compatible with volatile metabolites |
| • Relatively short time for sample analysis with sub-2 μm stationary phase particles |
• Novel compound identification is difficult |
| • Matching with multiple MS detectors |
| IMS |
• In situ detection |
• Ion suppression |
| • Providing location information of metabolites |
• Imaging quality is affected by resolution |
| • Relatively long time for sample analysis |
