Fig. 4. The association of rHDL with leukocytes in HWB assessed by flow cytometry detecting the fluorophore label (DiO) incorporated in the rHDL. (A) Histograms representing fluorescence intensity of DiO for each type of leukocytes for one of the donors. (B) The MFI for each cell type, subtracted background and adjusted by the integral of DiO absorbance from the SEC analysis for each rHDL formulation. (C) Distribution of leukocyte-associated rHDL, obtained using the eqn (1). The data in B and C represent mean + SEM from two experiments using HWB from different donors.
