A comparison of enzyme loading and activity of immobilized laccase on the MBC prepared by adsorption, precipitation and crosslinking methods (pH = 3.5, 25 °C).
Adsorption | Adsorption & precipitation | Adsorption, precipitation & crosslinking | |
---|---|---|---|
Laccase loading (mg Lac per mg MBC) | 0.027 | 0.048 | 1.424 |
Activity per MBC (U per mg MBC) | 0.045 | 0.082 | 2.251 |
Specific activity (U per mg protein) | 1.672 | 1.719 | 1.581 |