Fig. 6. Effect of nanoparticles loaded with MC and CAPE on the pathogenicity-related gene expression of R. solanacearum. Nanoparticles (4 mg mL⁻¹) loaded with MC and CAPE, 4 mg mL⁻¹ active pharmaceutical ingredient, and Triton X-100 were added to the bacterial suspension (OD₆₀₀ = 0.8–1) at a 2 : 1 volume ratio and incubated overnight at 30 °C for 24 h. These treatments were marked as NPs, API, and Triton groups, respectively. The untreated RS-5 bacterial suspension was set as the blank group. (A) The gene expression levels of pehC, pilT, and polA were obtained from R. solanacearum treated with the bacteriostatic agent for 24 h. (B) The gene expression levels of phcA, phcB, and egl were obtained from R. solanacearum treated with a bacteriostatic agent for 24 h. Error bars represent the standard error of at least three independent trials.