Fig 2. Angiogenesis in vitro of ECs of HD1.
Native (a-c), control (d-f) and knockdown group (g-i) including the eGFP control of SCR1 (dGFP, eGFP, fGFP) and sh1 (gGFP, hGFP, iGFP) at day 5 (a, d, dGFP, g, gGFP), 25 (b, e, eGFP, h, hGFP) and 50 (c, f, fGFP, i, iGFP) of cultivation are presented. Elongated and linearly arranged cells of stage 2–3 (a, d, g) in all groups at day 5, was followed in N1 and SCR1 by networking (b, e) and three-dimensional organisation of stage 5–6 (c, f). The knockdown group showed a decrease (h), followed by an increase in cell density, ending up with networking structures of stage 4 (i). Control group showed positive eGFP control during the investigation period (dGFP, eGFP, fGFP), whereas sh1 displayed a reduction in fluorescent signals (gGFP, hGFP, iGFP). Scale bars = 100 μm.