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. 2022 Apr 28;17(4):e0266774. doi: 10.1371/journal.pone.0266774

Fig 3. Angiogenesis in vitro of ECs of HD2.

Fig 3

Native (a-c), control (d-f) and knockdown group (g-i) of HD2 including eGFP control of SCR2 (dGFP, eGFP, fGFP) and sh2 (gGFP, hGFP, iGFP) at day 5 (a, d, dGFP, g, gGFP), 25 (b, e, eGFP, h, hGFP) and 50 (c, f, fGFP, i, iGFP) of cultivation. Elongated shaped cells of stage 2 (a, d, g) in all groups at day 5, was followed in N2 and SCR2 by networking (b, e) and three-dimensional organisation in stage 5–6 (c, f). The knockdown group showed a persistent decrease in cell density (h, i). Control group showed positive eGFP control during the investigation period (dGFP, eGFP, fGFP), whereas sh2 displayed a reduction in fluorescent signals (gGFP, hGFP, iGFP). Scale bars = 100 μm.