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. 2022 Apr 28;17(4):e0266774. doi: 10.1371/journal.pone.0266774

Fig 5. mRNA and protein expression of VIM, TPI and MAT2A in knockdown cells.

Fig 5

A. Median and standard error of relative VIM mRNA expression of sh1, SCR1, sh2, SCR2. VIM was significantly downregulated in sh1 and sh2 at each investigation day (p<0.001), except of day 50 in sh1. Statistical analysis was carried out using Mann–Whitney U test for unpaired data. B. Relative mRNA expression of TPI of sh1, SCR1, sh2, SCR2. TPI was significantly upregulated in sh1 at day 15 (p<0.05) and downregulated in sh2 at day 15 (p<0.001), and 25 (p<0.05). Statistical analysis was carried out using Mann–Whitney U test for unpaired data. C. Median and standard error of relative MAT2A mRNA expression of sh1, SCR1, sh2, SCR2. MAT2A was significantly upregulated in sh1 (p<0.01), and downregulated in sh2 at day 5 (p<0.001). Statistical analysis was carried out using Mann–Whitney U test for unpaired data. D. Western blot analysis of VIM, TPI and MAT2A in sh1, SCR1, sh2, SCR2 at day 5, 15, 25. ACT was used as internal control. *p<0.05, **p<0.01, ***p<0.001.