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. 2022 Apr 28;17(4):e0266889. doi: 10.1371/journal.pone.0266889

Table 3. False positive rate.

Solid tumor Mutations Number of Mutations Detected False Positive
1% 0.50% 0.125% 0%
A 50ng 37(100%) 36(97%) 26(70%) 0(0%)
10ng 37(100%) 36(97%) 23(62%) 1(3%)
B 50ng 38(100%) 38(100%) 38(100%) 0(0%)
10ng 38(100%) 38(100%) 33(87%) 0(0%)
C 50ng 38(100%) 37(97%) 34(89%) 1(3%)
10ng 38(100%) 37(97%) 23(61%) 0(0%)
* D 50ng 5(100%) 5(100%) 4(80%) 1(20%)
10ng 5(100%) 5(100%) 2(40%) 0(0%)
E 50ng 37(100%) 36(97%) 36(97%) 4(11%)
10ng 37(100%) 35(95%) 19(51%) 1(3%)
Myeloid Mutations Number of Mutations Detected False Positive
1% 0.50% 0.13% 0%
A 50ng 15(94%) 15(94%) 4(25%) 0(0%)
10ng 15(94%) 13(81%) 4(25%) 0(0%)
B 50ng 17(100%) 17(100%) 17(100%) 0(0%)
10ng 17(100%) 17(100%) 11(65%) 0(0%)
C 50ng 16(94%) 15(88%) 8(47%) 0(0%)
10ng 16(94%) 15(88%) 1(6%) 0(0%)
E 50ng 17(100%) 17(100%) 17(100%) 6(35%)
10ng 17(100%) 17(100%) 12(71%) 2(12%)

*5 reference mutations were covered by the assay