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. 2022 Apr 28;18(4):e1010167. doi: 10.1371/journal.ppat.1010167

Fig 4. S. Typhimurium chitinases do not modulate the innate immune response in vivo.

Fig 4

(A-L) mRNA expression of innate immune genes extracted from cecal tissue of S. Typhimurium infected mice 48 hpi. Expression is normalized to the housekeeping gene actb as well as gene expression in uninfected mice. WT n = 8, ΔSTM0233 n = 8, ΔchiA n = 8, ΔSTM0233 ΔchiA n = 4. Bars represent geometric mean ± geometric SD. (M) Histopathological scoring of cecal tissue from S. Typhimurium infected mice 48 hpi. WT n = 8, ΔSTM0233 n = 8, ΔchiA n = 8, ΔSTM0233 ΔchiA n = 9. Tissues were scored for submucosal edema, neutrophil infiltration, goblet cells, and epithelial integrity. Bars represent mean ± SD. (N) Representative images of hematoxylin & eosin stained cecal tissue from S. Typhimurium infected mice 48 hpi. 400x magnification was used for WT and ΔSTM0233 ΔchiA images, 200x magnification was used for ΔchiA and ΔSTM0233 images. Statistics: (A-M) Stars indicate significance compared to the WT control by one-way ANOVA with Dunnett’s multiple comparison test. * = p<0.05, ** = p<0.01.