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. 2022 Apr 28;18(4):e1010167. doi: 10.1371/journal.ppat.1010167

Fig 5. The presence of WT S. Typhimurium rescues the invasion of chitinase-deficient S. Typhimurium.

Fig 5

Mice infected by oral gavage with a 1:1 ratio of WT and chitinase-deficient S. Typhimurium. (A) S. Typhimurium colonies recovered from fecal samples collected at 8 and 24 hpi. Fecal samples at 48 hpi were collected directly from the lumen of the colon. Invasion of colonic tissue was determined with a gentamicin protection assay. (B) Luminal samples from the terminal ileum were collected 48 hpi to determine S. Typhimurium colonization. Invasion was determined with a gentamicin protection assay performed on the terminal ileum. (C) Colonization of the Peyer’s patches (PP), mesenteric lymph nodes (MLN), spleen, and liver 48 h after intragastric infection. n = 5. Top panels A-C: CFU/mg recovered for WT and chitinase-deficient S. Typhimurium. Bars represent geometric mean ± geometric SD. Bottom panels A-C: the same data expressed as a competitive index, CI = (WT colonization/mutant colonization)/ (WT inoculum/mutant inoculum). Bars represent geometric mean ± geometric SD. Statistics: (Top Row) Paired t-test was performed with the absolute values (Bottom Row) the competitive index was analyzed with a one-sample t-test against our null hypothesis (competitive index = 1). * = p<0.05.