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. 2022 Apr 28;18(4):e1010407. doi: 10.1371/journal.ppat.1010407

Fig 1. Chitinase deletion impairs bacterial invasion in human epithelial cells.

Fig 1

(A) chiA expression from STY WT, STY ΔchiA, STY ΔchiA:chiA isolated from infected Caco-2 cells 16 hpi. (B) % invasion, (C) intracellular proliferation of STM WT and STM ΔchiA strains, (D) % invasion and (E) Intracellular proliferation of STY WT, STY ΔchiA, STY ΔchiA:chiA and STY ΔchiA:pQE60 strains in Caco-2 cells by gentamicin protection assay. (N = 3, n = 3). Unpaired Student’s t test was used to analyze the data for B-C and one-way ANOVA was used to analyze the data for D-E. RNA expression level of SPI1 and SPI2 genes in from STM WT and STM ΔchiA during (F) early phase (2 hpi) and (G) late phase (16 hpi) of infection in Caco-2 cells. (N = 3, n = 3); Two-way ANOVA was used to analyze the data.