Skip to main content
. 2022 Apr 28;18(4):e1010407. doi: 10.1371/journal.ppat.1010407

Fig 6. Chitinase enhances Salmonella Typhi virulence in C. elegans.

Fig 6

(A) Bacterial fold change as the ratio of CFU obtained from the worms after 24 hours and 48 hours continuous feeding on STY WT, STY ΔchiA, STY ΔchiA:chiA and STY ΔchiA:pQE60 strains. (N = 4); One way ANOVA was used to analyze the data. (B) Survival of the worms fed on E. coli OP50, STY WT, STY ΔchiA and STY ΔchiA:chiA. Data are presented from one independent experiment, representative of 4 independent experiments (N = 4). (C) Representative images of bacterial colonization in worms gut as observed by infecting transgenic FT63 worms with mCherry expressing bacteria for the indicated time. Yellow arrows show the presence of intact bacteria in the terminal bulb of the worm. (D) Representative images of bacterial colonization on the chitin-rich organs of the worms as detected by eosin Y staining. Yellow arrows show colocalization of the bacteria (red) and the eosin-stained chitin-containing regions (green). White arrow shows the absence of colocalization of the bacteria and chitin-rich organs. (E) Representative images showing bacterial colonization and persistence in the worms’ gut.