Fig. 3.

Role of λ Red proteins in the efficient resolution of cointegrants.

A. Percentage of cointegrant resolution (EcN‐pGECfimEcN_gfp) after 5 h induction with L‐ara of bacteria carrying helper plasmids pACBSR, pACBS or pACBSG, as indicated. These helper plasmids express I‐SceI, whereas the presence of gam and beta/exo genes is indicated under each plasmid (+).

B. Viable bacteria after 5 h induction with L‐ara of EcN‐pGECfimEcN_gfp cointegrants carrying pACBSR, pACBS or pACBSG, as indicated. Viability was calculated as CFU per unit of OD600 of the cultures.

A and B. Horizontal lines indicate the mean of five independent experiments (individual dots, n = 5) and vertical bars indicate standard deviation.