Primers used in the qRT-PR.

Gene name	Nucleotide sequence	Melting temperatures (°C)	PCR product size (bp)
LGR5-sense	5′-AGAGCCTGATACCATCTGCAAAC-3′	61.9	1114
LGR5-antisense	5′-TGAAGGTCGTCCACACTGTTGC-3′	65.8
ASCL2-sense	5′-TTTCCTGTGCCGCACCAGAACT-3′	68.4	108
ASCL2-antisense	5′-CAGCGACTCCAGACGAGGTGG-3′	67.8
CDKN1-sense	5′-TCGCTGTCTTGCACTCTGGTGT-3′	65.7	630
CDKN1-antisense	5′-CCAATCTGCGCTTGGAGTGATAG-3′	65.3
Bmi1-sense	5′-ACTACACGCTAATGGACATTGCC-3′	62.4	222
Bmi1-antisense	5′-CTCTCCAGCATTCGTCAGTCCA-3′	65
Msi1-sense	5′-GTTCATCGGAGGACTCAGTTGG-3′	63.2	852
Msi1-antisense	5′-CTGGTCCATGAAAGTGACGAAGC-3′	65.3
EphB3-sense	5′-CCTGTGTCAAGATCGAGGAGGT-3′	62.9	217
EphB3-antisense	5′-CTTCAGCGTCTTGATAGCCACG-3′	64.4
Caspase3-sense	5′-GGAGTCTGACTGGAAAGCCGAA-3′	65.1	762
Caspase3-antisense	5′-CTTCTGGCAAGCCATCTCCTCA-3′	66.1
GAPDH-sense	5′-CATCACTGCCACCCAGAAGACTG-3′	66.4	153
GAPDH-antisense	5′-ATGCCAGTGAGCTTCCCGTTCAG-3′	68.9
Rictor-sense	5′-CAGTGTGAGGTCCTTTCCATCC-3′	63.1	400
Rictor-antisense	5′-GCCATAGATGCTTGCGACTGTG-3′	65.2
Rptor-sense	5′-AGAAGGGTCTCCAAGGACGACT-3′	62.7	1875
Rptor-antisense	5′-GCAGGACACAAAGGCAGCATTG-3′	67.3