Fig. 6. Histological assessment on the longitudinal sections of the spinal cords at 8 weeks after SCI. (A) H&E (left) and immunofluorenscence (right) staining for the longitudinal sections at low magnification; (B) is an enlarged view of local tissue stained with HE. (C) Is an immunofluorescence staining images: MAP2 (blue, a neuronal marker). The arrows pointed to the PCL. Immunofluorescence showed that axonal regeneration was more pronounced in group 3 and followed the PCL direction. (D) Quantification of the percentages of Tuj-1-positive neurons in the lesion area of the rats in each group. Data presented as mean ± standard deviation (n = 3 for each group). p values in (D) were determined by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test, * denotes p < 0.05, ** denotes p < 0.01.