TABLE 3.
Characterization of fluoroquinolone-resistant E. coli strainsa
| Strain | Topoisomerase mutations
|
AcrA overexpression | OSTb | ||
|---|---|---|---|---|---|
| GyrA | ParC | GyrBc | |||
| CH1 | S83L D87N | S80I | ND | − | − |
| CH2 | S83L D87N | S80I E84G | WT | − | − |
| CH3 | S83L D87N A93T | S80I | ND | + | + |
| CH4 | S83L D87N | S80I A108V | ND | − | − |
| CH5 | S83L D87N | S80I | ND | + | + |
| CH6 | S83L D87N | S80I | WT | + | + |
| CH7 | S83L D87N | S80I A108V | ND | + | + |
| CH8 | S83L D87N | S80I | ND | − | − |
| CH9 | S83L D87N A93T | E84K | ND | + | + |
| CH10 | S83L D87N | S80I E84G | WT | + | + |
| CH11 | S83L D87N A93T | S80I | ND | + | + |
| CH12 | S83L D87N | E84K | ND | + | + |
| CH13 | S83L D87N | S80I | ND | − | − |
| CH14 | S83L D87Y A93T | S80I | ND | − | − |
| CH15 | S83L D87N | S80I | ND | + | + |
| CH16 | S83L D87Y A93S | S80I | ND | − | − |
| CH17 | S83L D87N | S80I | ND | + | + |
| CH18 | S83L D87N | S80I | WT | − | − |
| CH19 | S83L D87N | E84K | ND | + | + |
| CH20 | S83L D87Y | S80I | ND | + | + |
| CH21 | S83L D87N A93T | S80I A108V | ND | + | + |
| CH22 | S83L D87N | S80I | ND | − | − |
| CH23 | S83L D87N | E84K | ND | + | + |
| CH24 | S83L D87N | S80I | WT | + | + |
| CH25 | S83L D87N | S80I | ND | − | + |
| CH26 | S83L D87N | E84K | ND | + | + |
| CH27 | S83L D87N | S80I | WT | + | + |
| CH28 | S83L D87N | S80I E84G | ND | − | − |
| CH29 | S83L D87N | S80I | ND | + | + |
| CH30 | S83L D87N | S80I | ND | + | + |
a
None of the strains showed overexpression of MarA or SoxS.
b
OST in hexane:cyclohexane (3:1).
c
WT, wild-type; ND, not determined.