Fig. 1. CircBANP was indeed circular transcripts and significantly up-regulated in GC. The expression of circBANP was assessed by qRT-PCR in 56 pairs GC tissues and adjacent normal gastric tissues (A and B), GC cell lines (MKN-45, MKN-74, AGS and HGC-27) and human normal gastric epithelial cell line GES-1 (C). (D) Divergent and convergent primers were used to amplify circBANP and linear BANP mRNA, respectively, with cDNA and gDNA from GC cells as templates. GAPDH was used as negative control. (E) Total RNA was extracted from MKN-74 and MKN-45 cells, and random and oligo-dT primers were used for reverse transcription, respectively, followed by the detection of linear transcripts and circular transcripts by qRT-PCR. (F) Total RNA was extracted from MKN-74 and MKN-45 cells, and then incubated with RNase R, followed by the measurement of linear transcripts and circular transcripts by qRT-PCR. *P < 0.05 vs. normal, total RNA or mock control.