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. 2022 Apr 14;13:850040. doi: 10.3389/fendo.2022.850040

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Copyright © 2022 On, Su, Shen, Arokiaraj, Cardoso, Li and Chow

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression profile of the PACAP/GCGa and PACAP/GCG-R transcripts in adult B. belcheri. (A) Representative image of a whole adult individual indicating the localization of the collected tissues for expression analysis. (B) Relative expression of PACAP/GCGa and PACAP/GCG-R in the head (without the gills), middle part (without hepatic caecum), posterior part (with hepatic caecum and midgut), gills, hepatic caecum and midgut, muscle, skin, notochord, anterior nerve (LCM), and Hatschek’s pit (LCM). Melting curves for each transcript in qRT-PCR are all a single peak. Data is expressed as means ± SEM (n = 6).