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. 2022 Apr 14;13:850040. doi: 10.3389/fendo.2022.850040

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Copyright © 2022 On, Su, Shen, Arokiaraj, Cardoso, Li and Chow

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of PACAP/GCGa in several adult B. belcheri tissues by immunofluorescence. Immunofluorescence digital images of DAPI staining (blue), PACAP/GCGa (red) positive signals, and merged and negative control (merged). Negative controls represent the result of incubations in which primary antibody with overnight incubation with antigen in 1:10 molar ratio was used to replace primary antibody alone. PACAP/GCGa peptides were detected in the gill, midgut, and hindgut. N, notochord; Hp, Hatschek’s pit. The scale bar is 50 μm and is indicated in the figures.