Some fluorescent probes previously reported for the detection of H2O2, O2− and 1O2 and their imaging applicationa.
| Probes | λ ex/λem (nm) | Response time (min) | Imaging application | Ref. |
|---|---|---|---|---|
| Detection of H 2 O 2 | ||||
| (DCFH-DA) | 488/525 | 20 | Eukaryotic culture cell | 40 |
| RhH2 | 507/529 | 30 | Eukaryotic culture cell | 41 |
| (DCM-B) | 557/688 | 30 | HepG2 cells | 42 |
| FE–H2O2 | 360/540 | 4 | HeLa cells | 55 |
| Detection of O 2 − | ||||
| DHE | 480/610 | At least 10 | Living cells | 43 |
| DPBF | UV 420 | 2 | Solution | 46 |
| HCy-ONO | 765/785 | 3 | Mice | 49 |
| HCy-SeH | 755/800 | 30 | Mice | 56 |
| Detection of 1 O 2 | ||||
| SOSG | 504/525 | 1 | Solution | 51 |
| ASG | 350/537 | 3 | Hela cells | 57 |
| MTTA-Eu3+ | 294/335 | 20 | Hela cells | 58 |
| FN-4 | 330/378 | No data | Solution | 59 |
a
DCFH-DA: 2,7-dichlorofluorescin diacetate; RhH2: dihydrorhodamines; DCM-B: (E)-2-(2-(4-((4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)oxy)styryl)-4H-chromen-4-ylidene) malononitrile; FE–H2O2: H2O2 reaction site and 4-ferrocenyl(vinyl)pyridine unit; DHE: dihydroethidine; DPBF: 1,3-diphenyl isobenzofuran; SOSG: 2,7-dichlorofluorescin diacetate; ASG: Aarhus Sensor Green; MTTA-Eu3+: [4′-(10-methyl-9-anthryl)-2,2′:6′,2′′-terpyridine-6,6′′-diyl]bis(methylenenitrilo) tetrakis-(acetic acid); FN-4: (E)-2-(2-(furan-2-yl)vinyl) naphtho[1,2-d]oxazole.