Fig. 4. Effects on tumor-infiltrating T cells. E.G7-OVA tumor-bearing mice (n = 4) were treated with vaccines. (A and B) Ratios of tumor-infiltrating CTLs (CD8⁺ cells) (A) and PD-1 expression on CTLs (B) were evaluated by flow cytometry. Data are shown as means ± SEM. *p < 0.05, **p < 0.01, by one-way ANOVA.