Table 2. Association with diet and weight, and heritability of the epigenetic readouts.

Type	EAA	Diet	Mean (SD)	Diet (p)	rBW0*	pBW0	rBWF*	pBWF	h2	Strain r†
Mouse clocks	EAA, pan	CD	–0.05 ± 0.21	<0.0001	0.19	0.006	0.29	<0.0001	0.49	0.54
		HFD	0.07 ± 0.21		0.21	0.01	0.42	<0.0001	0.50
	EAA, liver	CD	0 ± 0.17	ns	0.09	ns	0.20	0.003	0.40	0.73
		HFD	0.03 ± 0.14		0.22	0.01	0.49	<0.0001	0.52
	dev.EAA, pan	CD	–0.04 ± 0.23	0.004	0.09	ns	0.22	0.001	0.53	0.76
		HFD	0.03 ± 0.22		0.27	0.002	0.45	<0.0001	0.61
	dev.EAA, liver	CD	0 ± 0.2	ns	0.19	0.002	0.29	<0.0001	0.46	0.78
		HFD	0 ± 0.16		0.29	0.0007	0.47	<0.0001	0.60
	int.EAA, pan	CD	–0.05 ± 0.25	0.0003	0.03	ns	0.21	0.002	0.27	0.66
		HFD	0.06 ± 0.33		0.22	0.01	0.46	<0.0001	0.45
	int.EAA, liver	CD	–0.04 ± 0.22	<0.0001	0.05	ns	0.18	0.01	0.59	0.80
		HFD	0.11 ± 0.25		0.27	0.002	0.58	<0.0001	0.54
Entropy	-	CD	2.67 ± 0.02	ns	0.09	ns	0.05	ns	0.31	0.24(ns)
		HFD	2.67 ± 0.02		0.15	0.09	0.15	0.09	0.32

CD, control diet; HFD, high-fat diet; EAA, epigenetic age acceleration; int, interventional; dev, developmental.

^*BW0 is body weight at about 4.5 months of age (n = 339; 210 CD and 129 HFD); BWF is final weight at tissue collection (1 HFD case missing data; n = 338; 210 CD and 128 HFD).

^†Pearson correlation between strain means for n = 29 BXD genotypes kept on CD and HFD.