. 2020 Mar 5;10(16):9274–9289. doi: 10.1039/c9ra10765g

Experimental factorial design for the optimization of Bi₂O₃ NP production using nystatin drug (NS) and bismuth nitrate after exposure to gamma rays at 20.0 kGy, their wavelength (nm), and the corresponding optical density.

Runs	Nystatin (NS) (mg/10 ml)	Bismuth nitrate (mg/10 ml)	Ratio (mg; w/w)	Absorption of Bi₂O₃ NPs (O.D.)	Wavelength of Bi₂O₃ NPs (λ nm)
1	5.0	0.0	1 : 0	Control^a	Control^a
2	5.0	5.0	1 : 1	Nil^b	Nil^b
3	5.0	12.5	1 : 2.5	Nil^b	Nil^b
4	5.0	25.0	1 : 5	2.253	570.0
5	5.0	37.5	1 : 7.5	1.887	310.0
6	5.0	50.0	1 : 10	1.462	315.0
7	0.0	25.0	0 : 5	Control^a	Control^a
8	2.5	25.0	0.5 : 5	2.179	540.0
9	5.0	25.0	1 : 5	2.118	570.0
10	7.5	25.0	1.5 : 5	1.666	620.0
11	10.0	25.0	2 : 5	Nil^b	Nil^b
12	15.0	25.0	3 : 5	Nil^b	Nil^b

Control was used to autozero the results from the set of other experiments that did not contain one of the precursors used for Bi₂O₃ NPs-Nystatin synthesis.

Nil means there was no color change which confirms non-formation of Bi₂O₃ NPs-Nystatin.

Experimental factorial design for the optimization of Bi2O3 NP production using nystatin drug (NS) and bismuth nitrate after exposure to gamma rays at 20.0 kGy, their wavelength (nm), and the corresponding optical density.

Experimental factorial design for the optimization of Bi₂O₃ NP production using nystatin drug (NS) and bismuth nitrate after exposure to gamma rays at 20.0 kGy, their wavelength (nm), and the corresponding optical density.