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. 2022 Apr 21;2022:3447185. doi: 10.1155/2022/3447185

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Copyright © 2022 Yifei Fang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Predicted abundance of N1 TANs and N2 TANs quantified by flow cytometry. In each diagram, one solid black circle represents one population of cells, and the area of the circle correlates with the number of cells in that population. (a) Compared with the control group, the abundance of N1 (Ly6G+/CD206-) and N2 (Ly6G+/CD206+) cells in TME after gene knockout of TGF-β producing gene in myofibroblasts might not change significantly, and N2 (Ly6G+/CD206+) subgroup might be still more than the N1 (Ly6G+/CD206-) subgroup. After the addition of additional TGF based on the control group, the number of N2 (Ly6G+/CD206+) subgroup cells might further increase, and the number of N1 (Ly6G+/CD206-) subgroup cells might further decrease. The addition of 1D11 leads to increased N1 and decreased N2 population. (b) Compared with the control group, the number of N1 (Ly6G+/CD206-) subgroup significantly increases, and N2 (Ly6G+/CD206+) subgroup significantly decreases after the knockout of TGF-β producing gene in myofibroblasts. After the addition of additional TGF-β based on the control group, the number of N2 (Ly6G+/CD206+) subgroup cells might further increase, and the number of N1 (Ly6G+/CD206-) subgroup cells might further decrease. The addition of 1D11 results in expanded N1 and reduced N2. (c) There can be little difference between in vivo and in vitro controls. In vivo, compared with the control group, gene knockout of TGF-β producing gene in myofibroblasts might not affect the differentiation of N1 (Ly6G+/CD206-) and N2 (Ly6G+/CD206+). However, in vitro, gene knockout of TGF-β producing gene in myofibroblasts might result in a significant increase in the N1 (Ly6G+/CD206-) population and a significant decrease in the N2 (Ly6G+/CD206+) population. After the addition of additional TGF-β based on the control group, the number of N2 (Ly6G+/CD206+) subgroup cells might further increase, and the number of N1 (Ly6G+/CD206-) subgroup cells might decrease further. The addition of 1D11 promotes N1 and suppresses N2 differentiation.