Fig. 3. (a) Antiproliferative activity of compounds 1–7, 10, and 12 against the human TNBC MDA-MB-231 cells at 10 μM screening doses using the MTT assay. (b) Dose–response effects of various compound 1 treatments on the viability of the TNBC MDA-MB-231 cells compared with the DMSO vehicle control. The viable cell count was determined using the MTT assay. Error bars show the SEM of N = 3/dose, *p < 0.05 as compared with the vehicle-treated control groups. (−) Oleocanthal was used as a positive control at 10 μM. ^#p < 0.05 as compared with the (−)-oleocanthal treated control group. The IC₅₀ values are included for activity comparison.