Effects of enzyme amount and reaction time on the yield of 3a.

Entrya	Enzyme amount (mg)	Time (h)	Yieldb (%)
1	5	18	57
2	10	18	73
3	20	18	68
4	30	18	70
5	10	24	82
6	10	30	88
7	10	36	93
8	10	48	92

^aReaction conditions: TLIM, 4-chlorobenzaldehyde (1 mmol), 1H-indole (2 mmol), water (5 mL), 55 °C.

^bHPLC yield.