The optimized conditions for UHPLC-QE-MS mass spectrometry.
QE | Positive | Negative |
Spray voltage (kV) | 4.0 ESI+ | 3.6 ESI− |
Capillary temperature (°C) | 400 | 400 |
Sheath gas flow rate (Arb) | 45 | 45 |
Aux gas flow rate (Arb) | 15 | 15 |
Mass range (m/z) | 70–3000 | 70–3000 |
Full mass resolution | 70 000 | 70 000 |
MS/MS resolution | 17 500 | 17 500 |
TopN | 3 | 3 |
NCE/stepped NCE | 20, 40, 60 | 20, 40, 60 |