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. 2020 Mar 18;10(19):11264–11273. doi: 10.1039/d0ra00389a

The optimized conditions for UHPLC-QE-MS mass spectrometry.

QE Positive Negative
Spray voltage (kV) 4.0 ESI+ 3.6 ESI
Capillary temperature (°C) 400 400
Sheath gas flow rate (Arb) 45 45
Aux gas flow rate (Arb) 15 15
Mass range (m/z) 70–3000 70–3000
Full mass resolution 70 000 70 000
MS/MS resolution 17 500 17 500
TopN 3 3
NCE/stepped NCE 20, 40, 60 20, 40, 60